New Microscope Technology Captures Super-Resolution Images of Internal Cell Structures
A new experimental framework, named REID (Reaction-Enabled Imaging), has been developed to capture super-resolution images of internal cell structures at approximately 100 nanometers. This technology, detailed in Nature, utilizes the cells' own chemical glow rather than external lasers, which can be damaging. Researchers discovered that by swapping a common chemical co-reactant for a biological buffer called Bis-Tris, the cellular glow was boosted by 1,000 times, allowing for sharp, unblurred images in just 20 milliseconds. REID combines computer algorithms with electricity-triggered, chemical, and biological luminescence to enhance image clarity. It has successfully built a 3D map of a cell's skeleton, resolving structures 116 nanometers apart horizontally and 235 nanometers apart vertically, and has captured proteins on cell surfaces with high sensitivity. This method is significantly gentler on cells compared to traditional laser-based microscopes, enabling continuous, high-quality footage for extended ...