What's Happening?
A new protocol has been developed for using genetically encoded multichannel EMcapsulin reporters in both fluorescence microscopy and electron microscopy (EM). These EMcapsulins, which manifest as distinct
shapes in EM, provide comprehensive guidance for labeling cells or proteins with fluorescent markers. The protocol includes detailed instructions for biochemical quality control and can be adapted for custom EMcapsulin constructs tailored to specific experimental needs.
Why It's Important?
The use of multiplexed genetic tags in microscopy allows for more precise localization of proteins within cells, which is essential for understanding cellular functions and disease mechanisms. This advancement could significantly impact research in cell biology and pathology, providing researchers with tools to study complex cellular processes and interactions at a molecular level. It opens new avenues for drug development and disease treatment by enabling detailed visualization of cellular components.
