What's Happening?
BGI-Research has published a new spatial transcriptomics method, Stereo-seq V2, which achieves single-cell resolution on FFPE sections. This method enables simultaneous in situ profiling of host and microbial RNAs, overcoming previous limitations in analyzing degraded and crosslinked RNA in FFPE tissues. The technique combines deparaffinization/decrosslinking with random-primed capture and uniform gene-body coverage, opening new possibilities for research in infectious disease biology, tumor ecosystems, and clinical applications.
Why It's Important?
Stereo-seq V2 represents a significant advancement in spatial transcriptomics, particularly for FFPE samples, which are commonly used in clinical practice. By enabling high-resolution mapping of host and microbial transcriptomes, this method could enhance the understanding of complex biological interactions in diseases and improve diagnostic and therapeutic strategies. The ability to analyze FFPE samples at single-cell resolution could lead to breakthroughs in cancer research, infectious disease studies, and personalized medicine.
