What's Happening?
Researchers at Peking University have developed a novel method for spatial RNA imaging called 'profiling of RNA in situ through single-round imaging' (PRISM). This technique utilizes multiplex fluorescence in situ hybridization (FISH) with multichannel
color barcoding to distinguish dozens of RNA transcript species in a single imaging round. The method addresses the challenge of resolving multiple barcodes using only four spectral channels by employing a spectral barcoding scheme. Each targeted gene transcript is assigned a unique barcode on a padlock probe, with each segment of the barcode corresponding to a specific spectral channel. This allows for the encoding of distinct fluorescence intensity levels, significantly enhancing the capacity for RNA imaging.
Why It's Important?
The development of the PRISM method represents a significant advancement in the field of molecular biology and genetics. By enabling the simultaneous imaging of multiple RNA transcripts, this technique can provide deeper insights into cellular processes and gene expression patterns. It holds potential applications in various research areas, including cancer research, developmental biology, and neuroscience. The ability to visualize complex RNA interactions in situ can lead to a better understanding of disease mechanisms and the development of targeted therapies. This innovation could also accelerate research timelines and reduce costs associated with traditional imaging methods.
